商品介绍
Product details
Images
Application
ELISA, FC
Description
Recombinant protein rFel d 1 is expressed in Escherichia coli. DNA sequence encoding 175 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,4 kDa.
Contents
Recombinant proteinrFel d 1 - 1 vial (0.1 mg), lyophilized.Reconstitute with sterile deionized water with or without azide.
Preparation
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Formulation
Lyophilized from a 0.22 μm filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8), preservative- and carrier-free.Purity > 95% (HPLC method).
Storage and handling
Store the lyophilized product in freezer(-20°C to -80°C).Shipping and short term storage (max. 1 month) is possible at room temperature. The expiration date of unopened product vial is indicated on the vial label.After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer(-20°C to -80°C).Avoid freeze/thaw cycles.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Manufactured by Apronex Ltd., an EXBIO company.
ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rFel d 1 and allergen-specific human plasma IgG4 antibodies of Felis domesticus positive donor.A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rFel d 1.
Recombinant allergen rFel d 1 purity verification. 5 µg of rFel d 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
Flow cytometry dot-plot staining pattern of rFel d 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
品牌介绍
EXBIO是研究和临床应用单克隆抗体和试剂的领先制造商，专注于人类细胞标记物的多色流式细胞术分析。
我们拥有超过4000种现成产品的综合产品组合，涵盖血液学，免疫学，癌症，干细胞和细胞生物学等多个领域。凭借经验丰富的细胞计数专家和生物技术工程师，EXBIO为我们的客户提供了广泛选择的荧光色素结合物中经过长期验证的抗体标记。
我们对客户需求的承诺得到我们符合ISO 13485：2016，ISO9001：2015和cGMP（21 CFR part 820）的集成质量管理体系的支持，使我们能够完成我们的使命：
EXBIO努力超越客户最苛刻的期望通过以可承受的价格提供高质量产品和服务的全面组合，从而成为分析细胞仪领域的专家。
公司介绍
产品分类
Accessory reagents
Recombinant allergens
Antibodies
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