商品介绍
Product details
Images
Application
FC (QC tested)
Reactivity
Human
Description
The Lineage Cocktail FITC is designed to stain and gate-out human T lymphocytes (CD3+), B lymphocytes (CD19+CD20+), NK cells (CD16+CD56+), non-differentiated monocytes (CD14+), neutrophils (CD16+), and eosinophils (CD14+dim). Peripheral blood dendritic cells and basophils are Lineage negative and do not stain using this reagent. Whenever applicable, the lineage negative cells can be simultaneously stained by other monoclonal antibodies conjugated to appropriate fluorochromes. For example, dendritic cells can be distinguished by positive staining with anti HLA-DR, and basophils by positive staining with anti CD203c.
Contents
Antibody cocktail (1 ml, 50 tests) components: Anti human CD3 (clone MEM-57, IgG2a) FITCAnti human CD14 (clone MEM-15, IgG1)Anti human CD16 (clone LNK16, IgG1)Anti human CD19 (clone LT19, IgG1)Anti human CD20 (clone LT20, IgG2a)Anti human CD56 (clone MEM-188, IgG2a)Stabilizing buffer
Preparation
Premixed combination of mouse monoclonal antibodies labeled with FITC and diluted in optimal concentrations in stabilizing buffer
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Flow cytometry surface staining pattern of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry multicolor surface staining of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood) and anti-human CD203c (NP4D6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human target population cells (red-filled) from basophils (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Lineage Cocktail FITC (20 μl cocktail / 100 μl of peripheral whole blood).
品牌介绍
EXBIO是研究和临床应用单克隆抗体和试剂的领先制造商，专注于人类细胞标记物的多色流式细胞术分析。
我们拥有超过4000种现成产品的综合产品组合，涵盖血液学，免疫学，癌症，干细胞和细胞生物学等多个领域。凭借经验丰富的细胞计数专家和生物技术工程师，EXBIO为我们的客户提供了广泛选择的荧光色素结合物中经过长期验证的抗体标记。
我们对客户需求的承诺得到我们符合ISO 13485：2016，ISO9001：2015和cGMP（21 CFR part 820）的集成质量管理体系的支持，使我们能够完成我们的使命：
EXBIO努力超越客户最苛刻的期望通过以可承受的价格提供高质量产品和服务的全面组合，从而成为分析细胞仪领域的专家。
公司介绍
产品分类
Accessory reagents
Recombinant allergens
Antibodies
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