EXBIO/Recombinant human Cbp / PAG/0.1 mg/EXB0009,EXBIO,,EXBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：EXBIO/Recombinant human Cbp / PAG/0.1 mg/EXB0009

产品编号：EXB0009

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： EXBIO

公司：EXBIO

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商品介绍

Product details

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References

Application

Reactivity

Human

Description

Recombinant human PAG / Cbp is expressed in E. coli.DNA sequence encoding intracellular domain of human Cbp/PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.A calculated molecular mass of fussion protein is 45.3 kDa. As a result of highly acidic character of Cbp/PAG (predicted isoelectric point pI 4.4) and further modification by phosphorylation, the recombinant protein migrates as a 70 - 85 kDa protein in SDS-PAGE under reducing conditions.

Contents

Recombinant human PAG / Cbp - 1 vial (0.1 mg).Reconstitute vial content with 100 μl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

Preparation

The protein was purified by immobilized metal affinity chromatography used for purification of polyhistidine-tagged recombinant proteins, and lyophilized.

Formulation

Lyophilized from a 0.22 μm filtered solution in phosphate buffered saline (PBS).

Storage and handling

Store lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

SDS-PAGE Electrophoresis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). 12% separating gel, 6% stacking gel; reducing conditions.Lane 1-5: Cbp / PAG recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomassie Blue.

Western Blotting analysis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). Reducing conditions.Lane 1-4: immunostaining with anti-Cbp rabbit polyclonal antibody (EXBIO cat. no. 11-409-C100; dilution 0.5 μg/ml). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of Cbp/PAG recombinant protein loaded on each lane: (1) 100 ng, (2) 50 ng, (3) 20 ng, (4) 10 ng.

Product specific references:

Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.PubMed

品牌介绍

EXBIO是研究和临床应用单克隆抗体和试剂的领先制造商，专注于人类细胞标记物的多色流式细胞术分析。

我们拥有超过4000种现成产品的综合产品组合，涵盖血液学，免疫学，癌症，干细胞和细胞生物学等多个领域。凭借经验丰富的细胞计数专家和生物技术工程师，EXBIO为我们的客户提供了广泛选择的荧光色素结合物中经过长期验证的抗体标记。

我们对客户需求的承诺得到我们符合ISO 13485：2016，ISO9001：2015和cGMP（21 CFR part 820）的集成质量管理体系的支持，使我们能够完成我们的使命：

EXBIO努力超越客户最苛刻的期望通过以可承受的价格提供高质量产品和服务的全面组合，从而成为分析细胞仪领域的专家。

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