DescriptionRecombinant human TRAIL-R2 (DR5/ TNFRSF10B)/Fc Chimera is produced in HEK 293 cells. The extracellular domain of human TRAIL-R2 (aa 1 - 182) is fused to the Fc portion of human IgG1. A calculated molecular mass of fussion protein is 42 kDa. As a result of glycosylation, the recombinant protein migrates approximately as 45 kDa protein in SDS-PAGE under reducing conditions.
ContentsRecombinant human TRAIL-R2 (DR5/ TNFRSF10B)/Fc Chimera - 1 vial (0.05 mg). Reconstitute vial content with 50 μl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
PreparationThe protein was purified from cell culture supernatant by protein-A affinity chromatography and lyophilized.
FormulationLyophilized from a 0.22 μm filtered solution in phosphate buffered saline (PBS).
Storage and handlingStore lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.
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