EXBIO/Anti-Granzyme B FITC/100 tests/1F-634-T100,EXBIO,,EXBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：EXBIO/Anti-Granzyme B FITC/100 tests/1F-634-T100

产品编号：1F-634-T100

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： EXBIO

公司：EXBIO

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商品介绍

Product details

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References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

Human NK cell line YT-INDY-derived granzyme B

Other names

GZMB, HLP, CTLA1, SECT

Preparation

The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

Storage and handling

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden.Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor intracellular staining of human lymphocytes stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Martin P, Pardo J, Schill N, Joeckel L, Berg M, Froelich CJ, Wallich R, Simon MM: Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics. J Biol Chem. 2010 Apr 15. [Epub ahead of print] PubMed

Kummer JA, Kamp AM, Tadema TM, Vos W, Meijer CJ, Hack CE: Localization and identification of granzymes A and B-expressing cells in normal human lymphoid tissue and peripheral blood. Clin Exp Immunol. 1995 Apr;100(1):164-72. PubMed

Kummer JA, Wever PC, Kamp AM, ten Berge IJ, Hack CE, Weening JJ: Expression of granzyme A and B proteins by cytotoxic lymphocytes involved in acute renal allograft rejection. Kidney Int. 1995 Jan;47(1):70-7. PubMed

Product specific references:

Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin Exp Immunol. 1999 May;116(2):366-70.PubMed

Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.PubMed

Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus-induced IL-2 expression by central memory CD4+ T lymphocytes. J Immunol. 2005 Apr 15;174(8):4753-60.PubMed

Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160(7):3610-6.PubMed

品牌介绍

EXBIO是研究和临床应用单克隆抗体和试剂的领先制造商，专注于人类细胞标记物的多色流式细胞术分析。

我们拥有超过4000种现成产品的综合产品组合，涵盖血液学，免疫学，癌症，干细胞和细胞生物学等多个领域。凭借经验丰富的细胞计数专家和生物技术工程师，EXBIO为我们的客户提供了广泛选择的荧光色素结合物中经过长期验证的抗体标记。

我们对客户需求的承诺得到我们符合ISO 13485：2016，ISO9001：2015和cGMP（21 CFR part 820）的集成质量管理体系的支持，使我们能够完成我们的使命：

EXBIO努力超越客户最苛刻的期望通过以可承受的价格提供高质量产品和服务的全面组合，从而成为分析细胞仪领域的专家。

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