DescriptionRecombinant protein rVes v 2 is expressed in S2 cells (Drosophila).DNA sequence encoding 345 AAs was fused with Strep-tag at the N-terminus.A calculated molecular mass of recombinant protein is 40,44 kDa.
ContentsRecombinant proteinrVes v 2 - 1 vial (0.1 mg), lyophilized.Reconstitute with sterile deionized water with or without azide.
PreparationThe protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
FormulationLyophilized from a 0.22 μm filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8), preservative- and carrier-free.Purity > 95% (HPLC method).
Storage and handlingStore the lyophilized product in freezer(-20°C to -80°C).Shipping and short term storage (max. 1 month) is possible at room temperature. The expiration date of unopened product vial is indicated on the vial label.After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer(-20°C to -80°C).Avoid freeze/thaw cycles.
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